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doctoral thesis
The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa
Zagreb: University of Zagreb, School of Dental Medicine, 2017. urn:nbn:hr:127:980851
Knežević, Marjana
University of Zagreb
School of Dental Medicine
Department of Oral Medicine

Cite this document

Knežević, M. (2017). The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa (Doctoral thesis). Zagreb: University of Zagreb, School of Dental Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:127:980851

Knežević, Marjana. "The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa." Doctoral thesis, University of Zagreb, School of Dental Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:127:980851

Knežević, Marjana. "The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa." Doctoral thesis, University of Zagreb, School of Dental Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:127:980851

Knežević, M. (2017). 'The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa', Doctoral thesis, University of Zagreb, School of Dental Medicine, accessed 11 April 2025, https://urn.nsk.hr/urn:nbn:hr:127:980851

Knežević M. The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa [Doctoral thesis]. Zagreb: University of Zagreb, School of Dental Medicine; 2017 [cited 2025 April 11] Available at: https://urn.nsk.hr/urn:nbn:hr:127:980851

M. Knežević, "The role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa", Doctoral thesis, University of Zagreb, School of Dental Medicine, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:127:980851

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Metadata
TitleThe role of human papillomavirus (hpv) in the development of benign and malignant changes of the oral mucosa
Title (croatian)Uloga humanih papiloma virusa (hpv) u razvoju dobroćudnih i zloćudnih promjena sluznice usne šupljine
AuthorMarjana Knežević
MentorMarinka Mravak-Stipetić (mentor)
MentorMagdalena Grce (komentor)
Committee memberVlaho Brailo (predsjednik povjerenstva)
Committee memberIrina Filipović Zore (član povjerenstva)
Committee memberRomana Čeović (član povjerenstva)
Committee memberKrunoslav Kuna (član povjerenstva)
Committee memberIvan Alajbeg (član povjerenstva)
GranterUniversity of Zagreb
School of Dental Medicine
(Department of Oral Medicine)
Zagreb
Defense date and country2017-03-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Oral Medicine
Universal decimal classification
(UDC)		616.31 - Stomatology
Abstract
Objectives: The aim of this study was to determine the prevalence and type of human papillomavirus (HPV) in various oral lesions in comparison to control healthy oral mucosa, and the dynamic of HPV infection course (persistence, change of HPV type and clearance). Material and Methods: The study comprised 426 consented subjects, of which 343 patients with different oral lesions and 83 controls with apparently healthy oral mucosa, referred for oral examination in the period from 2000 to 2015. The... More diagnoses of oral lesions were established according to clinical criteria and confirmed by histopathology. Oral cytobrush samples were collected from oral lesions and healthy mucosa from different topographic sites and the HPV types were determined by polymerase chain reaction (PCR). The presence of HPV DNA was evaluated by consensus and type-specific (TS) primer-directed PCR. A subset of samples was analysed for the presence of both alpha and beta genus HPV types. Results: Out of 426 specimens, 144 (41.98%) from oral lesions and 15 (18.07%) controls were positive for HPV DNA; 22.3% of all tested samples contained beta-HPV type and 15.02% alpha-PV type. The highest prevalence of beta-HPV and high risk (HR) HPV (alpha-HPV) types were found in benign and premalignant oral lesions, while HR-HPVs were found in a small number of control samples. In 38 subjects sampling was performed more than twice, in average 2.29 times, with time distance between sampling of 5.8 months. Twenty-six of these 38 patients were continuously HPV negative, five had transient infection, five acquired a new HPV infection, one had persistent HPV infection, and one had initially co-infection with different HPV types followed with new one during which initial oral lesion became malignant. The topographic distribution of the alpha-PV and beta-PV types showed affinity for the anterior part of the oral cavity and particular risk localization. Conclusion: Positive finding of HPV in all age groups and on healthy oral mucosa, high prevalence of HPV types in oral premalignant and proliferative lesions, affinity for risk localization in the oral cavity, unpredictable dynamic course and change of HPV type indicates the need for regular oral assessment and HPV control in patients with initially positive findings on oral mucosa. Less
Abstract (croatian)
Cilj istraživanja bio je ispitati prevalenciju i tip humanog papiloma virusa (HPV) u različitim oralnim lezijama u usporedbi s kontrolnom, naizgled zdravom sluznicom i dinamiku tijeka HPV infekcije u ustima (ustrajnost, promjenu tipa HPV i prolaznost infekcije). Ispitanici i postupci: U ispitivanju je sudjelovalo 426 ispitanika, od kojih 343 bolesnika s različitim oralnim lezijama i 83 kontrolnih s naizgled zdravom sluznicom koji su upućeni na pregled usne šupljine u razdoblju od 2000. do... More 2015.godine. Svi ispitanici dali su svoj informirani pristanak prije uključivanja u ovo istraživanje. Dijagnoze oralnih lezija postavljene su na temelju kliničkih kriterija i potvrđene histopatološkom analizom. Citološki obrisci uzeti su citološkom četkicom s oralnih lezija i zdrave sluznice s topografski različitih mjesta sluznice, a HPV je dokazan lančanom reakcijom polimerazom (PCR). Prisutnost HPV DNA dokazana je PCR-om sa zajedničkim i tip-specifičnim početnicama. Određeni dio uzoraka bio je testiran na tipove HPV-a iz roda alfa i beta. Rezultati: U svih 426 testiranih uzoraka, HPV DNA dokazana je u 144 (41,98%) uzoraka s oralnih lezija i 15 (18,07%) kontrolnih; 22,3% ispitanih uzoraka sadržavalo je tip HPV iz roda beta, a 15,02% tip HPV iz roda alfa. Najveća prevalencija beta-HPV i visokorizičnih (HR) HPV tipova (alfa-HPV) pronađeni su u benignim i premalignim oralnim lezijama, a visokorizični HPV tipovi pronađeni su i u malom broju kontrolnih uzoraka. U 38 ispitanika uzorkovanje je provedeno više od dva puta, u prosjeku 2.29 puta, u vremenskom razmaku od prosječno 5,8 mjeseci. Dvadesetšest od 38 ispitanika bilo je stalno negativno na HPV, u pet je dokazana prolazna infekcija, pet je steklo novu infekciju HPV-om, jedan ispitanik imao je stalnu infekciju HPV-om, a jedan je tijekom praćenja početno dokazanu koinfekciju s različitim tipovima HPV-a i stekao novu s HPV tipom visokog rizika tijekom koje je inicijalna premaligna lezija sluznice postala zloćudna. Analiza topografske distribucije alfa-HPV i beta-HPV tipova na oralnoj sluznici pokazala je afinitet virusa za sluznicu prednjeg dijela usne šupljine i osobito za rizične lokalizacije. Zaključak: Pozitivan nalaz HPV-a u svim dobnim skupinama i na zdravoj sluznici te visoka prevalencija HPV tipova u oralnim premalignim i proliferativnim lezijama kao i afinitet za rizične lokalizacije te nepredvidljiv dinamičan tijek uz promjene tipa HPV-a upućuju na potrebu kontrole na HPV u kliničkoj stomatološkoj praksi kod inicijalno pozitivnog nalaza. Less
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:127:980851
Study programmeTitle: dental medicine
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina (doktor/doktorica znanosti, područje biomedicine i zdravstvo, polje dentalna medicina)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
RepositoryUniversity of Zagreb School of Dental Medicine Repository
Created on2018-01-11 11:31:09

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