Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population

Pavlinec, Željko

prikaz prve stranice dokumenta Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population

Embargo period 2023-07-12

doctoral thesis

Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population

Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:583354

Pavlinec, Željko

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Pavlinec, Ž. (2021). Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population (Doctoral thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:583354

MLA 8th Edition

Pavlinec, Željko. "Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:583354

Chicago 17th Edition

Harvard

Pavlinec, Ž. (2021). 'Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 September 2021, https://urn.nsk.hr/urn:nbn:hr:217:583354

Vancouver

Pavlinec Ž. Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2021 September 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:583354

IEEE

Ž. Pavlinec, "Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:583354

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:583354

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Metadata

Title	Assessment of MHC diversity in Central and Southeastern European grey wolf (Canis lupus) population
Title (english)	Procjena MHC raznolikosti u populacijama vuka (Canis lupus)¸srednje i jugoistočne Europe
Author	Željko Pavlinec MBZ: 42901040131
Mentor	Ana Galov (mentor)
Committee member	xxx xxx (predsjednik povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2021-07-12, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	57 - Biological sciences. Physical anthropology. Biodiversity
Abstract	Major histocompatibility complex diversity affects individual fitness, population viability and prospects for its long-term survival and can thereby serve as a valuable genetic indicator of population fitness and its ability to adapt to environmental changes. Ten populations of grey wolf (Canis lupus) have been identified in Europe today, the two largest being Carpathian and Dinaric-Balkan, with their sizes estimated to 4,000 and 5,000 individuals, respectively. In this thesis I assessed MHC class II allelic diversity in Central and Southeastern European grey wolves, namely from Carpathian and Dinaric Mountains. I used sequencing-cloning procedure to analyse exon 2 of DLA-DQA1, DQB1 and DRB1 genes in 99 samples from Carpathian population and 79 samples from Dinaric-Balkan population. I found four DQA, seven DQB and seven DRB alleles, with ten haplotypes in the Carpathian population, and eight DQA, 11 DQB and 12 DRB alleles, with 18 haplotypes in the Dinaric-Balkan population. Two alleles in Dinaric-Balkan population are unknown from previous research. There are strong indications of positive selection acting upon all examined loci in both populations, and a strong linkage disequilibrium between all pairs of loci. Dinaric-Balkan population seems to be the most diverse European population with regards to MHC genes.
Abstract (english)	Raznolikost glavnog sustava tkivne podudarnosti smatra se dobrim genetičkim pokazateljem fitnesa populacije i sposobnosti prilagodbe na promjene u okolišu, s obzirom da utječe na fitnes jedinke, vijabilnost populacije i njene izglede za dugoročno preživljavanje. Danas u Europi postoji deset populacija vuka (Canis lupus), od kojih su dvije najveće populacije karpatska i dinarsko-balkanska koje broje oko 4000, odnosno 5000 jedinki. U disertaciji je istražena raznolikost lokusa MHC klase II u populacijama vuka srednje i jugoistočne Europe, to jest populacija Karpatskog i Dinarskog gorja. Eksoni 2 lokusa DLA-DQA1, DQB1 i DRB1 analizirani su metodama sekvenciranja i molekularnog kloniranja u 99 uzoraka karpatske populacije i 79 uzoraka dinarsko-balkanske populacije. Identificirano je četiri DQA, sedam DQB i sedam DRB alela te deset haplotipova u karpatskoj populaciji, dok je u dinarskobalkanskoj populaciji identificirano osam DQA, 11 DQB i 12 DRB alela, te čak 18 haplotipova. Dva alela nađena u potonjoj populaciji nisu od ranije poznata. U obje populacije rezultati ukazuju na postojanje pozitivne selekcije na svim istraženim lokusima te na snažnu neravnotežu vezanosti alela između svih parova lokusa. Dinarsko-balkanska populacija pokazuje najvišu razinu genetičke raznolikosti procijenjenu na MHC lokusima od svih europskih populacija vukova.
Keywords
Keywords (english)
Language	english
URN:NBN	urn:nbn:hr:217:583354
Promotion	2021
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Embargoed access Embargo expiration date: 2023-07-12
Terms of use
Repository	Repository of Faculty of Science
Created on	2021-09-15 13:25:07

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