prikaz prve stranice dokumenta THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE
Embargo period 2026-04-02
doctoral thesis
THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:801886
Mirta, Tokić
University of Zagreb
Faculty of Science
Department of Biology

Cite this document

Mirta, T. (2024). THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:801886

Mirta, Tokić. "THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:801886

Mirta, Tokić. "THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:801886

Mirta, T. (2024). 'THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:801886

Mirta T. THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 March 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:801886

T. Mirta, "THE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:801886

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Metadata
TitleTHE ROLE OF de novo DNA METHYLATION IN PLANT RESPONSE TO ELEVATED TEMPERATURE
Title (croatian)ULOGA de novo METILACIJE DNA U ODGOVORU BILJAKA NA POVIŠENU TEMPERATURU
AuthorTokić Mirta
MentorNataša Bauer (mentor)
Committee memberMirta Tkalec (predsjednik povjerenstva)
Committee memberBranka Salopek-Sondi (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-04-02, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
One of the key factors affecting plant survival and agricultural yield production is temperature. In this thesis, effects of prolonged 37 °C and short-term 45 °C heat treatments were investigated in Arabidopsis thaliana, Brassica rapa ssp. pekinensis and Solanum lycopersicum. Elevated temperatures negatively affected germination, development, shoot and root growth, and flower morphology. Seedlings and inflorescences were more thermosensitive than leaves of adult plants. Global DNA methylation levels and expression of key RNA-directed DNA methylation (RdDM) genes varied in different species, tissues and under heat stress. To evaluate the role of de novo DNA methylation in heat stress, different A. thaliana lines with altered RdDM pathway were used. DNA methylation at single-base resolution in AGL14, FBW2 and RKP genes and expression of RdDM-related genes revealed differences under heat stress, indicating the importance of an intact de novo DNA methylation machinery in thermotolerance.
Abstract (croatian)
Temperatura je jedan od ključnih čimbenika koji utječu na preživljavanje biljaka i poljoprivredne prinose. U ovom doktorskom radu istražen je utjecaj dugotrajnog izlaganja biljaka Arabidopsis thaliana, Brassica rapa ssp. pekinensis i Solanum lycopersicum temperaturi od 37 °C i kratkotrajnog izlaganja na 45 °C. Povišene su temperature negativno utjecale na klijavost, razvoj, rast izdanaka i korijena te morfologiju cvijeta. Klijanci i cvatovi bili su osjetljiviji na toplinski stres od listova odraslih biljaka. Globalne razine metilacije DNA i ekspresija ključnih gena uključenih u metilaciju DNA posredovanu malim RNA molekulama (RdDM) varirala je u različitim vrstama i tkivima te nakon izlaganja toplinskom stresu. Za procjenu uloge de novo metilacije DNA u toplinskom stresu korištene su različite linije A. thaliana u kojima je promijenjen put RdDM. Promjene u metilaciji gena AGL14, FBW2 i RKP te ekspresiji gena uključenih u RdDM uzrokovane toplinskim stresom ukazuju na važnost intaktnog de novo mehanizma metilacije DNA u toleranciji na povišenu temperaturu.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:801886
Promotion2024
ProjectNumber: IP-2016-06-6229
Title: MATH-BTB proteini kao regulatori transkripcije i RNA posredovane metilacije DNA u biljnom razvitku
Title: MATH-BTB proteins as switches between transcription and RNA-directed DNA methylation during plant development
Acronym: PHYTOMETHDEV
Leader: Dunja Leljak-Levanić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
ProjectNumber: IP-2014-09-4359
Title: Fitohormoni u abiotskom stresu kupusnjača: mehanizam tolerancije i primjena
Title: Phytohormones in abiotic stress of Brassica crops: mechanism of tolerance and application
Acronym: PhytoBraCro
Leader: Branka Salopek-Sondi
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
ProjectNumber: KK.05.1.1.02.0005
Title: Agrobioraznolikost - osnova za prilagodbu i ublažavanje posljedica klimatskih promjena u poljoprivredi
Acronym: Agrobioclim
Leader: Smiljana Goreta Ban
Jurisdiction: Croatia
Funder: Europska komisija
ProjectNumber: Project-ID 57560291
Title: Uloga auksin amidohidrolaza uročnjaka (Arabidopsis thaliana) u toplinskom stresu
Leader: Jutta Ludwig-Müller i Branka Salopek Sondi
Jurisdiction: Croatia
Funder: DAAD i MZO
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-04-02
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RepositoryRepository of the Faculty of Science
Created on2024-04-15 11:09:03

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