In this dissertation, a universally applicable model of digital transformation of stakeholders in the maritime transport sector was created and validated. The model will help stakeholders shape more successful digital transformation strategies. First, bibliometric, content and thematic analyses of digitalization and digital transformation in maritime transport and seaports were performed using the CiteSpace and Leximancer tools. The research included various criteria, for example analysis by keywords, topics and research areas. The goal was to address fundamental research questions in order to define the key points in the current process of digitalization and digital transformation of maritime transport and seaports. The success factors that influence the digital transformation of stakeholders operating in the maritime transport sector, as well as drivers of digital transformation and barriers to its implementation, were then identified and analyzed. Drivers, success factors and barriers were grouped according to the Technology-Organization-Environment (TOE) framework, i.e., in three aspects: the technological aspect referring to the acceptance and implementation of modern technologies and innovations, their security and interoperability; the organizational aspect referring to organizational resources, organizational structure, communication among employees within the organization, and the aspect pertaining to the external environment which affects activities of the organization and its growth. Given the notable lack of literature on the topic of digital transformation in maritime transport and seaports, additional literature review and analysis were required before the research model could be created. For this reason, the digital transformation was analyzed from the perspective of three digital technologies and information systems: Maritime National Single Window, Blockchain and Port Community System. In addition, digital information services as drivers of digital transformation were analyzed. Then, qualitative methods were used to investigate the relevance of the factors, based on information obtained from experts in the maritime transport sector, who possess substantial experience in the field of digital transformation. In the last phase, the model was validated. For this purpose, the partial least squares structural equation modeling (PLS-SEM) approach was used. The research has shown that organizational, technological, and environmental factors affect the digitalization of the organizations in the maritime transport sector. As a result of digitalization, changes in business models are visible: II organizations in maritime transport sector generate additional revenue from new sources, provide new services, and introduce new sales channels. The contribution of this dissertation is manifold. First, the results of the dissertation can provide a basis for further research on digital transformation in the maritime transport sector. Second, the developed and validated universal digital transformation model may help stakeholders in the maritime transport sector to focus their resources more effectively and to shape more successful digital transformation strategies. The results can also help legislators in drafting more modern laws that will incorporate technological advances, resulting in better cooperation between stakeholders, simplification of business processes, better use of infrastructure and resources and sustainable business of stakeholders operating in the maritime transport sector.