The role of bridging fibers in the architecture of the metaphase  mitotic spindle

Polak, Bruno

prikaz prve stranice dokumenta The role of bridging fibers in the architecture of the metaphase mitotic spindle

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doctoral thesis

The role of bridging fibers in the architecture of the metaphase mitotic spindle

Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:642253

Polak, Bruno

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Polak, B. (2019). The role of bridging fibers in the architecture of the metaphase mitotic spindle (Doctoral thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:642253

MLA 8th Edition

Polak, Bruno. "The role of bridging fibers in the architecture of the metaphase mitotic spindle." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:642253

Chicago 17th Edition

Harvard

Polak, B. (2019). 'The role of bridging fibers in the architecture of the metaphase mitotic spindle', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 31 March 2023, https://urn.nsk.hr/urn:nbn:hr:217:642253

Vancouver

Polak B. The role of bridging fibers in the architecture of the metaphase mitotic spindle [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2023 March 31] Available at: https://urn.nsk.hr/urn:nbn:hr:217:642253

IEEE

B. Polak, "The role of bridging fibers in the architecture of the metaphase mitotic spindle", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:642253

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:642253

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Metadata

Title	The role of bridging fibers in the architecture of the metaphase mitotic spindle
Title (croatian)	Uloga premošćujućih vlakana u arhitekturi metafaznoga diobenoga vretena
Author	Bruno Polak
Mentor	Inga Marijanović (mentor)
Committee member	Juraj Simunić (predsjednik povjerenstva)
Committee member	Marin Barišić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2019, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	577 - Biochemistry. Molecular biology. Biophysics
Abstract	Mitosis is a fundamental process during which the genetic material is transferred to daughter cells and is orchestrated by the mitotic spindle. Between two opposite spindle poles microtubules form bundles that interact with chromosomes. Bundles that bind to kinetochores on the chromosome are called kinetochore fibers, whilst certain microtubules form antiparallel overlap bundles in the central part of the spindle. With this research localization and distribution of antiparallel overlap bundles in metaphase and the spatial organization of these bundles and kinetochore fibers possess will be investigated. By using confocal microscope whole metaphase spindles will be imaged which will enable to determine the number of overlap regions and their association with kinetochore fibers. Further work will be focused on understanding the spatial organization of the whole mitotic spindle. Used approach revealed that nearly all antiparallel fibers are linked with kinetochore fibers and that human mitotic spindle is a chiral structure.
Abstract (croatian)	Mitoza je fundamentalan process kojim se genetička informacija prenosi na stanice kćeri, a struktura koja upravlja ovim procesom je diobeno vreteno, Između dva nasuprotna pola vretena pružaju se vlakna mikrotubula koja stvaraju interakcije sa kromosomima. Vlakna koja se vežu za kinetohore na kromosomima nazivaju se kinetohorna vlakna. Neki od ostalih svežnjeva stvaraju antiparalelne preklapajuće regije u centralnom djelu diobenog vretena. U ovom radu ispitati će se lokalizacija i zastupljenost antiparalelnih preklapajućih regija u metafazi te će se istražiti prostorna organizacija navedenih i kinethornih vlakana. Uz pomoć metode konfokalne mikroskopije snimati će se cijela vretena što će omogućiti utvrđivanje broja antiparalelnih svežnjeva, a odgovoriti će na pitanje njihove povezanosti sa kinetohornim vlaknima. Daljnji rad biti će usmjeren na razumijevanje prostorne organizacije metafaznog diobenog vretena. Uz primjenu navedenog pristupa, otkriveno je da su antiparalelna vlakna povezana sa kinetohornim vlaknima te da je diobeno vreteno u ljudskim stanicama kiralna struktura.
Keywords
Keywords (croatian)
Language	english
URN:NBN	urn:nbn:hr:217:642253
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2019-07-16 09:40:27