Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea

Mucko, Maja

prikaz prve stranice dokumenta Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea

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doctoral thesis

Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea

Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:928047

Mucko, Maja

University of Zagreb
Faculty of Science

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APA 6th Edition

Mucko, M. (2018). Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:928047

MLA 8th Edition

Mucko, Maja. "Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:928047

Chicago 17th Edition

Harvard

Mucko, M. (2018). 'Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 12 September 2024, https://urn.nsk.hr/urn:nbn:hr:217:928047

Vancouver

Mucko M. Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 September 12] Available at: https://urn.nsk.hr/urn:nbn:hr:217:928047

IEEE

M. Mucko, "Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:928047

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:928047

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Metadata

Title	Morphology and phylogeny of picoeukaryotes and planktonic pennate diatoms in the middle and south Adriatic Sea
Title (croatian)	Morfologija i filogenija pikoeukariota i penatnih planktonskih dijatomeja u srednjem i južnom Jadranu
Author	Maja Mucko
Mentor	Zrinka Ljubešić (mentor)
Committee member	Sunčica Bosak (predsjednik povjerenstva)
Granter	University of Zagreb Faculty of Science Zagreb
Defense date and country	2018-07-06, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES
Abstract	The smallest protists, generally called picoeukaryotes, and planktonic pennate diatoms have received limited attention in world oceans, although they are numerous and important for primary production, biogeochemical cycles and for carbon injection into the deep ocean. In this thesis, represented through seven scientific publications, cultivated planktonic pennate diatoms and picoeukaryotes were investigated with traditional methods (light and electron microscopy, morphometry) and with molecular analyses, including multi-gene phylogenies, and were identified as genera Entomoneis, Pseudo-nitzschia, Haslea and Picochlorum. Detailed investigations of genus Entomoneis resulted with description of seven new species. General picoeukaryotic diversity was addressed by eDNA amplicon sequencing V4 variable region of nuclear 18S rRNA gene. Obtained results identified 95% of the picoeukaryotic community as hetero- or mixotrophic, mostly belonging to parasitic dinoflagellates and radiolarians, while only 5% of the community was represented with photoautotrophic picoeukaryotes belonging to classes of green algae, haptophytes, stramenopiles and cryptophytes. One cultivated Picochlorum sp. strain was subjected to growth rate experiment and pigment and lipid analyses, resulting in interesting and promising data for future biotechnological studies.
Abstract (croatian)	Najmanji protisti, poznati kao pikoeukarioti i penatne planktonske diatomeje često su zanemareni u istraživanjima svjetskih oceana, iako predstavljaju brojnu i važnu komponentu za primarne proizvodnje, biogeokemijskih ciklusa i značajni su za protok ugljika u duboki ocean. U sklopu ove disertacije kroz sedam znanstvenih publikacija navedene skupine organizama istraživane su tradicionalnim metodama (svjetlosna i elektronska mikroskopija, morfometrija) i molekularnim analizama koje uključuju filogenetske anlize sa više različitih genskih markera te su identificirane kao rodovi Entomoneis, Pseudo-nitzschia, Haslea i Picochlorum. Detaljna analiza roda Entomoneis rezultirala je opisom sedam novih vrsta za znanost. Bioraznolikost ukupne pikoeukariotske zajednice u južnom Jadranu odredila se sekvenciranjem amplikona okolišne DNA i to prema varijabilnoj V4 regiji nuklearnog 18S rRNA gena. Dobiveni rezultati identificirali su 95% pikoeukariotske zajednice kao hetero- ili miksotrofne organizme, većim dijelom pripadajući parazitskim dinoflagelatima i radiolarijama, dok je samo 5% zajednice zastupljeno fotoautotrofnim pikoeukariotima koji pripadaju razredima zelenih algi, haptofita, stramenopila i kriptofita. Jedan kultivirani soj alge Picochlorum sp. istražen je kroz eksperiment određivanja brzine rasta te analizama pigmenta i lipida, što je rezultiralo zanimljivim i obećavajućim rezultatima koji mogu poslužiti kao okosnica za buduće biotehnološke studije.
Keywords
Keywords (croatian)
Language	english
URN:NBN	urn:nbn:hr:217:928047
Study programme	Title: Biology Study programme type: university Study level: postgraduate
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2018-09-04 11:32:21