THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY

Štimac, Valentina

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doctoral thesis

THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY

Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:130520

Štimac, Valentina

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Štimac, V. (2024). THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:130520

MLA 8th Edition

Štimac, Valentina. "THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:130520

Chicago 17th Edition

Harvard

Štimac, V. (2024). 'THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:130520

Vancouver

Štimac V. THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:130520

IEEE

V. Štimac, "THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:130520

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:130520

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Title	THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY
Title (croatian)	ULOGE AUGMINA I AKTINA UNUTAR DIOBENOG VRETENA U KONGRESIJI KROMOSOMA, ARHITEKTURI VRETENA I VJERNOSTI MITOZE
Author	Valentina Štimac
Mentor	Iva Marija Tolić (mentor)
Committee member	Andreja Ambriović Ristov (predsjednik povjerenstva)
Committee member	Maja Matulić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2024-10-23, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	576 - Cellular and subcellular biology. Cytology
Abstract	The human mitotic spindle is made of microtubules nucleated at centrosomes, at kinetochores, and from pre-existing microtubules by the augmin complex. Recently, spindle actin also emerged as a possible player in spindle assembly. However, it is unknown how augmin-mediated nucleation affects distinct microtubule classes, what the spatial distribution of spindle actin and microtubules is, and, finally, what the roles of these two proteins are in spindle architecture and mitotic progression. Here, by using superresolution microscopy and cutting edge live-cell imaging, I found that augmin promotes mitotic fidelity by generating assemblies consisting of bridging and kinetochore fibers that align sister kinetochores to face opposite poles, thereby preventing erroneous attachments. Furthermore, I showed that spindle actin, by forming fine filaments in the polar region and along spindle microtubules, promotes spindle pole focusing and bipolar cell division.
Abstract (croatian)	Mikrotubuli diobenog vretena se u ljudskim stanicama nukleiraju na centrosomu, kinetohorama te na površini već postojećih mikrotubula pomoću augminskog kompleksa. Nedavno je predloženo da i aktin unutar diobenog vretena ima ulogu u njegovom sastavljanju. Međutim, još uvijek nije poznato na koji način nukleacija pomoću augmina utječe na različite klase mikrotubula, koja je prostorna raspodjela aktina i mikrotubula unutar vretena te koja je uloga ova dva proteina u arhitekturi vretena i progresiji mitoze. Koristeći superrezolucijsku mikroskopiju te najnoviju tehnologiju snimanja živih stanica pokazala sam da augmin ima ulogu u pravilnoj podjeli kromosoma. Augmin stvara sklopove mikrotubula koji se sastoje od premošćujućih i kinetohornih vlakana što poravnava sestrinske kinetohore na način da su okrenute prema suprotnim polovima, te tako sprječava pogrešna vezanja s mikrotubulima. Nadalje, pokazala sam da aktin unutar vretena promiče fokusiranje polova vretena te bipolarnu mitozu tako što formira filamente u polarnoj regiji te uzduž mikrotubula.
Keywords
Keywords (croatian)
Language	english
URN:NBN	urn:nbn:hr:217:130520
Promotion	2024
Project	Number: PZS-2019-02-7653 Title: Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena Title: Mechanisms of microtubule bundle formation required for spindle maturation Acronym: SpindleForm Leader: Iva Marija Tolić Jurisdiction: Croatia Funder: Hrvatska zaklada za znanost Funding stream: Programme Research Cooperability
Study programme	Title: Doctoral study Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2024-12-02 18:11:40