Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva

Mahmuljin, Irma

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doctoral thesis

Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva

Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:883218

Mahmuljin, Irma

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Mahmuljin, I. (2015). Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva (Doctoral thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:883218

MLA 8th Edition

Mahmuljin, Irma. "Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:883218

Chicago 17th Edition

Harvard

Mahmuljin, I. (2015). 'Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 30 March 2023, https://urn.nsk.hr/urn:nbn:hr:217:883218

Vancouver

Mahmuljin I. Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2023 March 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:883218

IEEE

I. Mahmuljin, "Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:883218

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:883218

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Metadata

Title	Protuglikanska protutijela i glikozilacija imunoglobulina g u raku debeloga crijeva
Title (english)	Anti-glycan antibodies and glycosylation of immunoglobulin g in colorectal cancer
Author	Irma Mahmuljin
Mentor	Gordan Lauc (mentor)
Committee member	Olga Gornik (član povjerenstva)
Committee member	Biljana Balen (član povjerenstva)
Committee member	Aleksandar Vojta (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2015, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	57 - Biological sciences. Physical anthropology. Biodiversity
Abstract	Promjene glikozilacije su nerijetko opisivane u različitim fiziološkim i patološkim stanjima, te kao posljedica stila života. Promjene u metabolizmu tumorskih stanica rezultiraju proizvodnjom izmijenjenih glikanskih struktura, koje imunosni sustav prepoznaje i generira nova protuglikanska protutijela. Prisustvo i probir ovih protutijela je analiziran pomoću glikanskih čipova, a glikozilacija imunoglobulina G (IgG) pročišćavanjem iz plazme, i kromatografskom analizom glikana. Rezultati istraživanja su pokazali da postoje značajne razlike između zdravih osoba i oboljelih od raka. Vezanje protutijela je dalo pozitivne rezultate na 24 od 48 glikanskih struktura uz značajnu statističku značajnost između izučavanih grupa. Analiza glikozilacije IgG-a je pokazala da 10 od 23 kromatografska vrška mijenjaju udio unutar kromatograma u raku debeloga crijeva, a promjene galaktozilacije su jedan od pokazatelja pojave i napretka bolesti. Razumijevanje promjena glikozilacije imunoglobulina G i interakcija koje se događaju između ovih proteina i ugljikohidrata, a koje su izražene u različitim bolesnim stanjima, važno je za razumijevanje biologije tumorskih stanica.
Abstract (english)	Glycosylation changes are often described in different physiological processes and pathological conditions and as a lifestyle effect. Alterations in cancer cells metabolism result in the production of altered glycan structures, which are being recognized by the immune system that result in generation of novel anti-glycan antibodies. Presence and screening of antibodies was performed using glycan arrays. Immunoglobulin G (IgG) glycosylation was performed using plasma purification and glycan chromatography. Results show significant differences between healthy individuals and those with cancer. Antibody binding to 24/48 glycans give positive results with great statistical significance between studied sample groups. IgG glycosylation analysis shows that 10/23 glycan chromatographic peaks are changed in colorectal cancer and that galactosylation is one of the main indicators of appearance and progression of the disease. Understanding of IgG glycosylation changes and protein-glycan interactions, and the fact they are effective in numerous diseases are important for understanding biology of cancer cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:883218
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-05-17 09:15:45