Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci

Jurica, Karlo

prikaz prve stranice dokumenta Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci

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doctoral thesis

Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci

Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:488435

Jurica, Karlo

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Jurica, K. (2016). Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:488435

MLA 8th Edition

Jurica, Karlo. "Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:488435

Chicago 17th Edition

Harvard

Jurica, K. (2016). 'Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:488435

Vancouver

Jurica K. Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:488435

IEEE

K. Jurica, "Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:488435

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:488435

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Metadata

Title	Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci
Title (english)	Phenolic compounds in strawberry tree (Arbutus unedo L.) and their biological effects
Author	Karlo Jurica
Mentor	Dario Kremer (mentor) MBZ: 240541
Mentor	Vesna Benković (sumentor)
Committee member	Domagoj Đikić (član povjerenstva)
Committee member	Sandra Radić Brkanac (član povjerenstva) MBZ: 245550
Committee member	Nevenka Kopjar (član povjerenstva) MBZ: 195102
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2016, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	577 - Biochemistry. Molecular biology. Biophysics
Abstract	U ovom su radu ispitane fitokemijske značajke, antimikrobna svojstva i biološki učinci lista obične planike. Određen je sadržaj ukupnih fenola, flavonoida, tanina, fenolnih kiselina, te polifenolni profil lista UHPLC-MS/MS (Orbitrap) metodom, sadržaj arbutina i hidrokinona HPLC-UV/DAD metodom, antioksidacijska svojstva FRAP, TEAC i DPPH metodama. MIK koncentracije su utvrđene dvostrukom mikrodilucijom, a mutagenost komet-testom i CBMN testom. Dokazano je 37 novih fenolnih spojeva. Sadržaj arbutina u listu bio je 0,3 %, dok hidrokinon nije detektiran. Metanol je bolje sredstvo za ekstrakciju polifenola od vode. Metanolni ekstrakt lista pokazao je bolji antioksidacijski, te antimikrobni učinak na ATCC i kliničke sojeve mikroorganizama. Arbutin nije pokazao antimikrobni učinak, dok je njegov metabolit hidrokinon pokazao pozitivan učinak na ispitivane uropatogene. U uvjetima in vitro i in vivo vodeni ekstrakt lista pri koncentraciji arbutina koja odgovara maksimalno dopuštenom dnevnom unosu ima nisku genotoksičnost. Potencijal vodenog ekstrakta lista obične planike kao fitoterpeutika za kliničku primjenu je velik.
Abstract (english)	This thesis investigated the phytochemical characteristics, antimicrobial properties and biological effects of strawberry tree leaves. Total phenol, tannin, flavonoid and phenolic acid content was determined as well as polyphenolic profile using the UHPLC-MS/MS Orbitrap method. Arbutin and hydroquinone concentrations were measured by HPLC-UV/DAD, antioxidative properties by FRAP, TEAC, DPPH, MIC concentrations by a Twofolded Microdilution method and mutagenicity by Comet-test and CBMN test. Thirty-seven new phenolic compounds were detected. Leaves contained 0.3% of arbutin while hydroquinone was not detected. Methanol was shown to be a more effective solvent for the extraction of polyphenols. Methanolic extract showed better antioxidative properties and better antimicrobial effect on standard ATCC and clinical microorganism strains. Arbutin did not show antimicrobial activity, while its metabolite hydroquinone showed positive antimicrobial activity against the tested uropathogens. Water extract applied at an arbutin concentration corresponding to the maximum allowable daily intake showed low genotoxicity in vitro and in vivo. Strawberry tree water leaf extract has great potential as a phytotherapeutic in clinical application.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:488435
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-05-16 08:45:52

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