prikaz prve stranice dokumenta DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)
Embargo period 2023-09-29
doctoral thesis
DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:718540
Filek, Klara
University of Zagreb
Faculty of Science
Department of Biology

Cite this document

Filek, K. (2022). DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA) (Doctoral thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:718540

Filek, Klara. "DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:718540

Filek, Klara. "DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:718540

Filek, K. (2022). 'DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 01 February 2023, https://urn.nsk.hr/urn:nbn:hr:217:718540

Filek K. DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA) [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2023 February 01] Available at: https://urn.nsk.hr/urn:nbn:hr:217:718540

K. Filek, "DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:718540

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Metadata
TitleDIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)
Title (croatian)RAZNOLIKOST MIKROBNIH ZAJEDNICA DIJATOMEJA I BAKTERIJA GLAVATIH ŽELVI (CARETTA CARETTA)
AuthorKlara Filek
MentorSunčica Bosak (mentor)
Committee memberTomislav Ivanković (predsjednik povjerenstva)
Committee memberZrinka Ljubešić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-11-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Loggerhead sea turtles are ancient marine reptiles inhabiting the oceans worldwide. They are colonized by microorganisms both in the gastrointestinal tract and on their carapace and skin. In this thesis that consists of four scientific publications, the loggerhead sea turtle-associated diatom and bacterial communities were investigated by high-throughput sequencing of chloroplast rbcL gene and 16S rRNA gene, and cultivation. The culture-independent approach has shown that loggerheads harbor diverse but stable bacterial communities in their cloaca, and that the oral microbiota reflects the turtle’s environment. The carapace and skin harbor complex microbial communities often rich in as yet unclassified taxa. Diatoms isolated from carapace and skin were cultivated as xenic monocultures and further characterized using morphology and molecular techniques to elucidate their identity and phylogeny. Novel diatom taxa were discovered, often belonging to the genera Craspedostauros, Fallacia, or Amphora genus. Cultivated diatoms also enabled the characterization of the phycosphere and isolation of diatom-associated bacterial strains.
Abstract (croatian)
Glavate želve su drevni morski gmazovi koji nastanjuju svjetska mora i oceane. Njihov probavni sustav te oklop i koža kolonizirani su mikroorganizmima. U ovoj disertaciji čiji su rezultati predstavljeni u sklopu četiri znanstvene publikacije, mikrobne zajednice dijatomeja i bakterija glavatih želvi su analizirane koristeći molekularne metode sekvenciranja visoke protočnosti kloroplastnog rbcL gena i 16S rRNA gena te kultiviranjem. Rezultati metoda neovisnih o kultivaciji su pokazali da su bakterijske zajednice u kloaki glavate želve raznolike ali stabilne te da oralna mikrobiota reflektira okoliš u kojem se kornjača nalazi. Karapaks i koža podloga su složenim mikrobnim zajednicama u kojima su često prisutni još uvijek nepoznati mikroorganizmi. Dijatomeje izolirane s karapaksa i kože kultivirane su kao ksenične monokulture te su korištene morfološke i molekularne metode za njihovu identifikaciju i određivanje filogenije. Otkrivene su nove svojte dijatomeja unutar rodova Craspedostauros, Fallacia i Amphora. Kultivacija dijatomeja također je omogućila analizu fikosfere te izolaciju pripadajućih bakterijskih sojeva.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:718540
Promotion2022
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2023-09-29
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2022-12-06 12:11:36

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