DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED
 WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)

Filek, Klara

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doctoral thesis

DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)

Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:718540

Filek, Klara

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Filek, K. (2022). DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA) (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:718540

MLA 8th Edition

Filek, Klara. "DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)." Doctoral thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:718540

Chicago 17th Edition

Harvard

Filek, K. (2022). 'DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 27 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:718540

Vancouver

Filek K. DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA) [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2025 March 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:718540

IEEE

K. Filek, "DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:718540

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:718540

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Title	DIVERSITY OF DIATOM AND BACTERIAL COMMUNITIES ASSOCIATED WITH LOGGERHEAD SEA TURTLES (CARETTA CARETTA)
Title (croatian)	RAZNOLIKOST MIKROBNIH ZAJEDNICA DIJATOMEJA I BAKTERIJA GLAVATIH ŽELVI (CARETTA CARETTA)
Author	Klara Filek
Mentor	Sunčica Bosak (mentor)
Committee member	Tomislav Ivanković (predsjednik povjerenstva)
Committee member	Zrinka Ljubešić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2022-11-29, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	57 - Biological sciences. Physical anthropology. Biodiversity
Abstract	Loggerhead sea turtles are ancient marine reptiles inhabiting the oceans worldwide. They are colonized by microorganisms both in the gastrointestinal tract and on their carapace and skin. In this thesis that consists of four scientific publications, the loggerhead sea turtle-associated diatom and bacterial communities were investigated by high-throughput sequencing of chloroplast rbcL gene and 16S rRNA gene, and cultivation. The culture-independent approach has shown that loggerheads harbor diverse but stable bacterial communities in their cloaca, and that the oral microbiota reflects the turtle’s environment. The carapace and skin harbor complex microbial communities often rich in as yet unclassified taxa. Diatoms isolated from carapace and skin were cultivated as xenic monocultures and further characterized using morphology and molecular techniques to elucidate their identity and phylogeny. Novel diatom taxa were discovered, often belonging to the genera Craspedostauros, Fallacia, or Amphora genus. Cultivated diatoms also enabled the characterization of the phycosphere and isolation of diatom-associated bacterial strains.
Abstract (croatian)	Glavate želve su drevni morski gmazovi koji nastanjuju svjetska mora i oceane. Njihov probavni sustav te oklop i koža kolonizirani su mikroorganizmima. U ovoj disertaciji čiji su rezultati predstavljeni u sklopu četiri znanstvene publikacije, mikrobne zajednice dijatomeja i bakterija glavatih želvi su analizirane koristeći molekularne metode sekvenciranja visoke protočnosti kloroplastnog rbcL gena i 16S rRNA gena te kultiviranjem. Rezultati metoda neovisnih o kultivaciji su pokazali da su bakterijske zajednice u kloaki glavate želve raznolike ali stabilne te da oralna mikrobiota reflektira okoliš u kojem se kornjača nalazi. Karapaks i koža podloga su složenim mikrobnim zajednicama u kojima su često prisutni još uvijek nepoznati mikroorganizmi. Dijatomeje izolirane s karapaksa i kože kultivirane su kao ksenične monokulture te su korištene morfološke i molekularne metode za njihovu identifikaciju i određivanje filogenije. Otkrivene su nove svojte dijatomeja unutar rodova Craspedostauros, Fallacia i Amphora. Kultivacija dijatomeja također je omogućila analizu fikosfere te izolaciju pripadajućih bakterijskih sojeva.
Keywords
Keywords (croatian)
Language	english
URN:NBN	urn:nbn:hr:217:718540
Promotion	2022
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Open access Embargo expiration date: 2023-09-29
Terms of use
Repository	Repository of the Faculty of Science
Created on	2022-12-06 12:11:36