Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo

Mužinić, Vedran

prikaz prve stranice dokumenta Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo

doctoral thesis

Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo

Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:461703

Mužinić, Vedran

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Mužinić, V. (2021). Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:461703

MLA 8th Edition

Mužinić, Vedran. "Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo." Doctoral thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:461703

Chicago 17th Edition

Harvard

Mužinić, V. (2021). 'Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:461703

Vancouver

Mužinić V. Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:461703

IEEE

V. Mužinić, "Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:461703

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:461703

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Title	Genotoksični učinci insekticida klorpirifosa, imidakloprida i α-cipermetrina i herbicida terbutilazina, glifosata i tembotriona u uvjetima in vitro i in vivo
Title (english)	Genotoxic effects of insecticides chlorpyrifos, imidacloprid and α-cypermethrin and herbicides terbuthylazine, glyphosate and tembotrione in vitro and in vivo
Author	Vedran Mužinić
Mentor	Davor Želježić (mentor)
Committee member	Vesna Benković (predsjednik povjerenstva)
Committee member	Mirjana Pavlica (član povjerenstva)
Committee member	Feodora Stipoljev (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2021-01-09, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	57 - Biological sciences. Physical anthropology. Biodiversity
Abstract	Pesticidi su iznimno važni kemijski spojevi, međutim, odavno je poznato da štetno djeluju i na neciljane organizme, uključujući i čovjeka. Genotoksičnost se očituje remećenjem strukture i funkcije DNA te je povezana s razvojem malignih bolesti. Stoga smo odlučili istražiti genotoksični učinak često korištenih pesticida u niskim razinama od značaja za ljudsku izloženost. Genotoksičnost insekticida klorpirifosa, imidakloprida i α-cipermetrina te herbicida glifosata, terbutilazina i tembotriona u uvjetima in vitro smo ispitali alkalnim komet testom, mikronukleus testom i analizom razdiobe kromosoma 18, 9, X i Y. Genotoksičnost α-cipermetrina i tembotriona u uvjetima in vivo ispitali smo alkalnim komet testom na gravidnim i dojećim štakoricama soja Wistar i njihovim neizravno izloženim mladuncima. Pesticidi su u uvjetima in vitro djelovali izraženije aneugenim mehanizmom. Tembotrion je pokazao izraženiji genotoksični učinak u odnosu na α-cipermetrin u odraslim štakoricama. Povišenje oštećenja DNA u mladuncima upućuje na mogući prijelaz oba pesticida preko placente, te tembotriona preko majčinog mlijeka.
Abstract (english)	Pesticides are exceptionally important chemical compounds, however, it has been known they can have harmful effects on non-target species, including humans. Genotoxicity is manifested by disruption of structure and function of DNA, and is associated with development of malign disesases. Therefore, we decided to examine the genotoxic effect of commonly used pesticides at low levels of importance to human exposure. We analyzed the in vitro genotoxicity of insecticides chlorpyrifos, imidacloprid, α-cypermethrin and herbicides gylphosate, terbuthylazine and tembotrione via alkaline comet assay, micronucleus assay as well as chromosome 18, 9, X, and Y segregation analysis. We analyzed the genotoxicity of α-cypermethrin and tembotrione in vivo by alkaline comet assay in gestating and lactating Wistar rat dams as well as their indirectly exposed offspring. The pesticides have effected mostly by aneugenic mechanism in vitro. Tembotrione showed more pronounced genotoxic effect compared to α-cypermethrin in dams. Elevation of DNA damage in offspring suggests possible transplacental transfer for both pesticides, as well as translactational transfer for tembotrione.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:461703
Promotion	2021
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2021-02-24 12:37:58

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