prikaz prve stranice dokumenta Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics
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doctoral thesis
Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:625111
Rudan, Marina
University of Zagreb
Faculty of Science
Department of Biology

Cite this document

Rudan, M. (2018). Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:625111

Rudan, Marina. "Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:625111

Rudan, Marina. "Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:625111

Rudan, M. (2018). 'Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:625111

Rudan M. Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:625111

M. Rudan, "Crosstalk of gene polymorphisms with RNA and protein phenotypic characteristics", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:625111

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Metadata
TitleCrosstalk of gene polymorphisms with RNA and protein phenotypic characteristics
Title (croatian)Povezanost genskih polimorfizama s fenotipskim karakteristikama molekula RNA i proteina
AuthorMarina Rudan
MentorMiroslav Radman (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberDavor Zahradka (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Both RNA and protein molecules can misfold into non-functional conformations. Since DNA is transcribed into RNA to direct the biosynthesis of proteins, functional cells require the maintenance of DNA, RNA and protein homeostasis. Here, it is explored whether DNA polymorphisms can impact upon RNA and protein conformation to the extent of displaying phenotypic changes. It has been found that this is a multifaceted process. Mutation-induced protein conformational changes can modify protein interactions and determine protein susceptibility to oxidation. Moreover, it was showed that deleterious mutations can be buffered at the transcript level with RNA chaperones (DEAD-box RNA helicases and cold shock proteins) acting as phenotypic suppressors of mutations and enhancing cellular fitness. These results also highlight the importance of mRNA quality control in eukaryotes and its potential to modulate cellular metabolic activity and lifespan. This research underscores the importance of studying systems-level crosstalk among DNA, RNA and protein maintenance, rather than studying individual processes separately.
Abstract (croatian)
Molekule RNA i proteini se mogu pogrešno smotati u nefunkcionalne konformacije. Budući da se za biosintezu proteina DNA prepisuje u RNA, funkcionalne stanice zahtijevaju održavanje homeostaze DNA, RNA i proteina. U ovom radu je istraženo mogu li DNA polimorfizmi utjecati na konformaciju molekule RNA i proteina, te rezultirati fenotipskim promjenama. Otkriveno je da je ovo iznimno složen proces, tijekom kojeg konformacijske promjene proteina izazvane mutacijama molekula DNA mogu modificirati interakcije proteina i odrediti njihovu osjetljivost na oksidaciju. Također, pokazano je da štetne mutacije mogu biti puferirane na razini transkripcije pomoću RNA šaperona (DEAD-box RNA helikaza i 'cold shock' proteina), koji djeluju kao fenotipski supresori mutacija i poboljšavaju stanični fitnes. Ovi rezultati naglašavaju značaj kontrole kvalitete mRNA u eukariotima i njen potencijal za modulaciju stanične metaboličke aktivnosti te životnog vijeka. Ovo istraživanje ističe važnost proučavanja cjelokupnog sustava DNA, RNA i proteina, te njihove međusobne komunikacije umjesto analize staničnih procesa pojedinačno.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:625111
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2018-04-06 10:08:29

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