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doctoral thesis
Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:994118
Musladin, Sanja
University of Zagreb
Faculty of Science
Department of Chemistry

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Musladin, S. (2014). Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:994118

Musladin, Sanja. "Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:994118

Musladin, Sanja. "Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:994118

Musladin, S. (2014). 'Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:994118

Musladin S. Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:994118

S. Musladin, "Uloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:994118

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Metadata
TitleUloga kompleksa RSC u remodeliranju strukture kromatina promotora PHO gena kvasca Saccharomyces cerevisiae
Title (english)The role of RSC complex in chromatin structure remodeling of the yeast Saccharomyces cerevisiae PHO gene promoters
AuthorSanja Musladin
MBZ: 304755
MentorSlobodan Barbarić (mentor)
Committee memberIta Gruić Sovulj (predsjednik povjerenstva)
Committee memberSlobodan Barbarić (član povjerenstva)
Committee memberMary Sopta (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2014-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Promotor gena PHO5 kvasca S. cerevisiae predstavlja jedan od najbolje istraženih modelnih sustava u proučavanju regulacije transkripcije remodeliranjem strukture kromatina. Ispitan je učinak inaktivacije katalitičkih podjedinica svih remodelirajućih kompleksa ne-esencijalnih za rast stanica kvasca na remodeliranje strukture kromatina promotora PHO5 te do danas nije nađen kompleks čija aktivnost bi bila neophodno potrebna za ovaj proces. Uloga kompleksa RSC, jedinog esencijalnog kompleksa uključenog u remodeliranje strukture kromatina u kvascu, u indukciji PHO promotora in vivo do sada nije ispitana. U ovom radu pokazano je da potpuna inaktivacija kompleksa RSC sprječava promjenu strukture kromatina prilikom indukcije promotora PHO5. Detaljnim kontrolnim eksperimentima je potvrđeno da je aktivnost ovog kompleksa izravno uključena u proces remodeliranja strukture kromatina promotora, odnosno da pokazani učinak nije posljedica zaustavljanja staničnog ciklusa ili slabije ekspresije nekog od proteina signalnog puta uslijed inaktivacije kompleksa. Za razliku od promotora PHO5, nađeno je da aktivnost kompleksa RSC nije uključena u remodeliranje strukture kromatina promotora PHO8 i PHO84, unatoč aktivaciji istim specifičnim aktivatorom transkripcije.
Abstract (english)
The yeast S. cerevisiae PHO5 promoter has proven an xcellent model for studying the regulation of gene expression through chromatin structure remodeling. The complete set of yeast remodeling complexes non-essential for cell viability was tested for involvement in massive transition of promoter chromatin structure upon induction, but none of the many tested remodeler gene mutations prevented PHO5 promoter opening. The role of RSC, only remodeling complex essential for cell viability, in chromatin remodeling upon induction of PHO promoters was unassessed in vivo so far. Here, the involvement of RSC complex was examined and it was shown that complete inactivation of RSCs activity prevented chromatin transition at the PHO5 promoter. A set of control experiments confirmed a direct effect of RSC inactivation, at the level of promoter chromatin remodeling, rather than being a consequence of compromised signaling pathway or cell cycle arrest. Interestingly, RSC was hardly involved in chromatin transitions at the PHO8 and PHO84 promoters, despite cofactor recruitment by the same transactivator.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:994118
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent111 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2017-02-15 13:50:54

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