Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani

Fredotović, Željana

prikaz prve stranice dokumenta Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani

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doctoral thesis

Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani

Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:480919

Fredotović, Željana

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Fredotović, Ž. (2017). Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:480919

MLA 8th Edition

Fredotović, Željana. "Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:480919

Chicago 17th Edition

Harvard

Fredotović, Ž. (2017). 'Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:480919

Vancouver

Fredotović Ž. Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:480919

IEEE

Ž. Fredotović, "Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:480919

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:480919

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Metadata

Title	Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani
Title (english)	Genome structure, chemical composition and biological activity of triploid onion Allium × cornutum Clementi ex Visiani
Author	Željana Fredotović
Mentor	Jasna Puizina (mentor) MBZ: 171072
Committee member	Mirjana Pavlica (član povjerenstva)
Committee member	Kroata Hazler-Pilepić (član povjerenstva) MBZ: 201953
Committee member	Vlatka Zoldoš (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2017, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	57 - Biological sciences. Physical anthropology. Biodiversity
Abstract	Allium × cornutum Clementi ex Visiani jedinstvena je forma luka, koja se pod nazivom 'Ljutika' tradicionalno uzgaja i koristi u ljudskoj prehrani u obalnoj i otočnoj Hrvatskoj. Otporna je na sušu i hladnoću, te prilagođena oskudnom, kamenitom tlu. Ovim radom željela se detaljnije istražiti struktura genoma te vrste, rasvijetliti nutritivni sastav te provjeriti njezinu biološku aktivnost. Provedene molekularno-filogenetske analize ribosomskih gena, 18S-5.8S-26S rDNA i 5S rDNA, otkrile su da je A. × cornutum (2n = 3x = 24) jedinstveni hibrid triju vrsta lukova: crvenog luka, Allium cepa L. i dva azijska luka, Allium pskemense B. Fedtsch i Allium roylei Stearn. Po jedan haploidni genom (x = 8) svake od triju roditeljskih vrsta identificiran je i vizualiziran na mitotskim i mejotičkim kromosomima A. × cornutum metodom fluorescentne hibridizacije in situ. Analize kloroplastnih i mitohondrijskih gena, pokazuju da A. × cornutum posjeduje jedinstveni tip sterilne citoplazme CMS-S, važne u oplemenjivanju lukova. Kemijske analize metanolnih ekstrakta A. × cornutum i A. cepa izvršene metodom HPLC-a, ukazuju na povećan sadržaj flavonoida (flavonola i antocijanina) u A. × cornutum, što može biti objašnjenje i nešto boljeg bioaktivnog profila triploida u odnosu na A. cepa. Obje vrste pokazuju značajnu antioksidativnu, antigenotoksičnu i antiproliferacijsku aktivnost, što ih potvrđuje kao važan izvor korisnih fitonutrijenata koji mogu doprinijeti zaštiti i očuvanju ljudskog zdravlja.
Abstract (english)	Allium × cornutum Clementi ex Visiani is unique onion type, traditionally cultivated in South and coastal Croatia under the name 'Ljutika' and used in human diet. It is resistant to cold weather conditiones and drought, and adapted to growth on poor stony ground. This paper attempts to explore genome structure, shed light on the nutritional composition and check biological activity of this species. Molecular-cytogenetic and phylogenetic analysis of two ribosomal genes, 18S-5.8S-26S rDNA and 5S rDNA revealed that 'Ljutika' is a unique hybrid of three onion species: common onion, Allium cepa L., and two Asian onions, Allium pskemense B. Fedtsch and Allium roylei Stearn. One haploid genome (x = 8) of each of the three parent species was identified and visualized on mitotic and meiotic chromosomes of A. × cornutum by fluorescent in situ hybridization. Chloroplast and mitochondrial gene analysis showed that 'Ljutika' possesses a sterile CMS-S type of cytoplasm, important for onion breeding. Phytochemical analysis determined by HPLC showed that methanolic extracts of A. × cornutum and A. cepa showed slightly higher content of flavonoids (flavonols and anthocyanins) in A. × cornutum, which may be cause of its higher bioactive profile in compare to A. cepa. Both species showed a significant antioxidant, antigenotoxic and antiproliferative activity which makes them an important sources of phytonutrients that can contribute to the protection and preservation of human health.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:480919
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-07-20 09:16:31

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