Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom

Martinić, Roko

prikaz prve stranice dokumenta Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom

Access restricted to students and staff of home institution

doctoral thesis

Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom

Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:257223

Martinić, Roko

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Martinić, R. (2014). Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:257223

MLA 8th Edition

Martinić, Roko. "Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:257223

Chicago 17th Edition

Harvard

Martinić, R. (2014). 'Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:257223

Vancouver

Martinić R. Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:257223

IEEE

R. Martinić, "Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:257223

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:257223

Please login to the repository to save this object to your list.

Metadata

Title	Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom
Title (english)	Biological effects of D- met-enkephalin isomer in a murine model of chemical liver injury induced by paracetamol
Author	Roko Martinić
Mentor	Nikola Štambuk (mentor) MBZ: 160463
Committee member	Mirko Hadžija (član povjerenstva) MBZ: 15065
Committee member	Gordana Lacković-Venturin (član povjerenstva)
Committee member	Ranko Stojković (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2014, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Universal decimal classification (UDC)	577 - Biochemistry. Molecular biology. Biophysics
Abstract	Peptidi i proteini u sisavaca i viših organizama građeni su od L-amino kiselina. Istraživanja peptida-enantiomera i upotreba D-amino kiselina je važan postupak za pripravu novih proteinskih struktura s različitim svojstvima u odnosu na nativne L-oblike. L-met-encefalin enantiomer posjeduje hepatoprotektivne odlike u mišjem modelu hepatotoksičnosti inducirane paracetamolom. Trenutno nema dostupnih podataka o osobinama i biološkim učincima Dmet- encefalin enantiomera, te je svrha ovog rada istražiti njegov hepatoprotektivni potencijal. D-enantiomer pokazao je zaštitni učinak u rasponu doza od 0,5 mg/kg do 20 mg/kg. Plazmatske aktivnosti enzima alanin aminotransferaza i aspartat aminotransferaza te nekroza jetre bile su značajno smanjene u usporedbi s kontrolnim životinjama koje su tretirane fiziološkom otopinom (p<0,01). Najjači potektivni učinak je uočen u životinja tretiranih s dozom od 5 mg/kg. Učinci su također ispitani u uvjetima blokade receptora i peptida. Pokazano je da su vjerojatno posredovani putem ζ i δ opioidnih receptora, a blokira ih naltrekson i antisens peptid IPPKY. Zaključeno je da su protektivni učinci D-met-encefalina usporedivi s učincima L-met-encefalina.
Abstract (english)	Peptides and proteins in mammals and higher organisms are composed of L-amino acids. The investigation of peptide-enantiomerism and the use of D-amino acids is important for the design of novel protein structures, with properties different from the native all L-forms. Lmet- enkephalin enantiomer exhibits strong protection in the mice model of acetaminophen induced hepatotoxicity. Howerver, there is currently no data available on the properties and biological effects of D-met-enkephalin enantiomer, and consequently we evaluated its hepatoprotective potential. D-enantiomer was shown to be protective in the dose range from 0.5 mg/kg to 20 mg/kg. Plasma AST, ALT and liver necrosis were significantly reduced in comparison to control animals treated with physiological saline (p>0.01). The best protection was observed in animals treated with a dose of 5 mg/kg. The effects of D-met-enkephalin were investigated from the standpoint of the receptor and peptide blockade. It was shown that they were probably mediated via δ and ζ opioid receptors, and blocked by naltrexone and antisense peptide. It is concluded that D-met-enkephalin induced hepatoprotection is comparable to L-met-enkephalin one.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:257223
Study programme	Title: Biology Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-05-18 10:16:46

Nacionalna i sveučilišna knjižnica u Zagrebu

Dabar