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doctoral thesis
Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:036239
Radić, Božo
University of Zagreb
Faculty of Science
Department of Biology

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Radić, B. (2016). Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:036239

Radić, Božo. "Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:036239

Radić, Božo. "Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:036239

Radić, B. (2016). 'Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:036239

Radić B. Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:036239

B. Radić, "Neuroleptička svojstva urapidila u uvjetima in vitro i in vivo", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:036239

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Metadata
TitleNeuroleptička svojstva urapidila u uvjetima in vitro i in vivo
Title (english)Neuroleptic properties of urapidil in condition in vitro and in vivo
AuthorBožo Radić
MentorPredrag Sikirić (mentor)
MBZ: 117454
MentorMaja Jazvinšćak Jembrek (sumentor)
MBZ: 233712
Committee memberxxx xxx (predsjednik povjerenstva)
MBZ: xxx
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Istražena su neuroleptička svojstva urapidila u štakora u uvjetima in vitro i in vivo. Urapidil je antihipertenziv, agonist centralnih 5-HT1a receptora i antagonist perifernih alfa 1 receptora s utjecajem na dopaminergičku transmisiju. Na stanicama PC12 i SH-SY5Y urapidil u dozi od 100 i 200 µM ne djeluje antioksidacijski (za razliku od kvercetina), niti zaustavlja smrt stanica izazvanu metamfetaminom (kao i haloperidol), ali dovodi do zaustavljanja aktivnosti efektorskih kaspaza 3/7 mitohondrijskog puta. In vivo u dozi od 0.25, 0.5 i 1 mg/štakoru intraperitonealno (i.p.), urapidil dovodi do povećanja lokomocije, bez izazivanja abnormalnih kretnji ili stereotipija karakterističnih za metamfetamin. Doze od 15, 20 i 25 mg/štakoru i.p. dovode do progresivne katalepsije, dok doza od 25 mg/štakoru i.p. urapidila u predtretmanu i posttretmanu zaustavlja hiperlokomociju i stereotipije izazvane metamfetaminom. Urapidil blokira aktivnost kaspaza 3/7 nakon primjene metamfetamina i iskazuje jasna neuroleptička svojstva, vjerojatno vežući se za dopaminske (D2) receptore.
Abstract (english)
Neuroleptic properties of urapidil were investigated in vitro and in vivo models in rats. Urapidil is an antihypertensive agent, agonist of central 5-HT1a and antagonist of peripheral alpha 1 receptors, with the effect on dopaminergic transmission. On PC12 and SH-SY5Y cells urapidil at doses of 100 and 200 µM is not acting like antioxidant (like quercetin does), nor stops cell death (like haloperidol) caused by methamphetamine, but blocks the activity of effector caspase 3/7 mitochondrial pathway. In vivo at a dose of 0.25, 0.5 and 1 mg/rat intraperitoneally (i.p.) it increases locomotion, but without causing abnormal movement or stereotypy like metamphetamine does. At doses of 15, 20 and 25 mg/rat i.p. urapidil causes progressive catalepsy. When applied as pretreatment and posttreatment it antagonizes metamphetamine induced hyperlocomotion and stereotypy. Urapidil blocks the activity of caspase 3/7 following administration of methamphetamine and expresses clear neuroleptic properties likely by binding to dopamine (D2) receptors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:036239
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2017-05-16 10:21:26

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