Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima

Lešić, Tina

prikaz prve stranice dokumenta Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima

doctoral thesis

Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:148695

Lešić, Tina

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Quality Control
Laboratory for Food Quality Control

Cite this document

APA 6th Edition

Lešić, T. (2022). Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:148695

MLA 8th Edition

Lešić, Tina. "Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:148695

Chicago 17th Edition

Harvard

Lešić, T. (2022). 'Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 October 2023, https://urn.nsk.hr/urn:nbn:hr:159:148695

Vancouver

Lešić T. Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2023 October 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:148695

IEEE

T. Lešić, "Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:148695

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:148695

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Title	Razvoj multimikotoksinske lc-ms/ms metode i molekularna identifikacija plijesni producenata citrinina, ciklopiazonične kiseline i sterigmatocistina u hrvatskim tradicionalnim mesnim proizvodima
Title (english)	Development of multimycotoxin lc-ms/ms method and molecular identification of mould producers of citrinin, cyclopiazonic acid and sterigmatocystin in Croatian traditional meat products
Author	Tina Lešić
Mentor	Jelka Pleadin (mentor)
Committee member	Ksenija Markov (predsjednik povjerenstva)
Committee member	Blaženka Kos (član povjerenstva)
Committee member	Ana Vulić (član povjerenstva)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Food Quality Control) (Laboratory for Food Quality Control) Zagreb
Defense date and country	2022-06-01, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Universal decimal classification (UDC)	58 - Botany
Abstract	Cilj rada bio je razviti i validirati multimikotoksinsku metodu tekućinske kromatografije u kombinaciji s masenom spektrometrijom (LC-MS/MS) za detekciju i kvantifikaciju mikotoksina značajnih za mesne proizvode: aflatoksin B1 (AFB1), okratoksin A (OTA), citrinin (CIT), sterigmatocistin (STC) i ciklopiazonična kiselina (CPA) te ispitati njihovu pojavnost u hrvatskim trajnim tradicionalnim mesnim proizvodima (TMP). Također, cilj je identificirati plijesni s površine TMP te ih dovesti u vezu s kontaminacijom mikotoksinima, uz dokazivanje prisutnosti toksikotvornih plijesni i gena odgovornih za njihovu biosintezu. Sa površine 250 analiziranih uzoraka TMP koji uključuju kobasice i suhomesnate proizvode uzorkovane tijekom 2 godine iz 5 regija Republike Hrvatske, ukupno je izolirano 535 izolata i identificirano 38 vrsta plijesni iz rodova Penicillium i Aspergillus. Identificirano je 9 različitih potencijalno toksikotvornih vrsta plijesni, od čega je 50 % potencijalnih AFB1 producenata (A. flavus), 58 % potencijalnih OTA producenata (P. nordicum, A. niger, A. westerdijkiae, A. welwitischie, A. tubingensis), 71 % potencijalnih CIT producenata (P. citrinum) te 48 % potencijalnih CPA producenata (P. commune, A. flavus, P. polonicum) imalo istraživane gene uključene u biosintezu mikotoksina. Razvijenom i validiranom visoko osjetljivom LC-MS/MS metodom odredile su se koncentracije mikotoksina STC do 3,93 μg/kg u 4 % uzoraka, OTA do 4,81 μg/kg u 10 % uzoraka te CPA do čak 335,5 μg/kg u 13 % uzoraka, dok AFB1 i CIT nisu detektirani u niti jednom uzorku TMP. U 21 % uzoraka kontaminiranih mikotoksinima, određeni su njihovi producenti. Pojavnost mikotoksina i površinskih plijesni dovedena je u vezu sa uvjetima proizvodnje TMP, uključujući tehnologiju i regionalne vremenske čimbenike.
Abstract (english)	The aim of this study was to develop and validate a multimycotoxin method of liquid chromatography with mass spectrometry (LC-MS /MS) for detection and quantification of mycotoxins important for meat products: aflatoxin B1 (AFB1), ochratoxin A (OTA), citrinin (CIT), sterigmatocystin (STC) and cyclopiazonic acid (CPA) and to investigate into their occurrence in Croatian dry-fermented traditional meat products (TMP). Also, the aim is to identify TMP surface molds and link them to the mycotoxin contamination, while proving the presence of toxigenic molds and genes responsible for their biosynthesis. From the surface of 250 analyzed samples including sausages and cured meat products sampled during 2 years from 5 Croatian regions, a total of 535 isolates and 38 species of molds of the genera Penicillium and Aspergillus were isolated. Nine different potentially toxigenic mold species were identified, of which 50% of the potential AFB1 producers (A. flavus), 58% of potential OTA producers (P. nordicum, A. niger, A. westerdijkiae, A. welwitischi A tubingensis), 71% of potential CIT producers (P.citrinum) and 48% of potential CPA producers (P.commune, A. flavus, P. polonicum) had the investigated gene involved in mycotoxin biosynthesis. With the developed and validated highly sensitive LC-MS/MS method, concentrations of STC up to 3.93 μg/kg in 4% of samples, OTA up to 4.81 μg/kg in 10% of samples, and CPA up to 335.5 μg/kg in 13% of samples were detected, while AFB1 and CIT weren't detected in any TMP sample. In 21% of mycotoxin contaminated samples, their producers were identified. The occurrence of mycotoxins and surface molds has been linked to TMP production conditions including technology and regional weather factors.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:148695
Promotion	2022
Study programme	Title: Postgraduate PhD Study in Nutrition Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
Type of resource	Text
Extent	195 str. ; 30 cm
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2022-11-09 14:23:08