Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka

Božić Luburić, Đurđica

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doctoral thesis

Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:409424

Božić Luburić, Đurđica

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

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APA 6th Edition

Božić Luburić, Đ. (2022). Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:409424

MLA 8th Edition

Božić Luburić, Đurđica. "Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:409424

Chicago 17th Edition

Harvard

Božić Luburić, Đ. (2022). 'Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 September 2023, https://urn.nsk.hr/urn:nbn:hr:159:409424

Vancouver

Božić Luburić Đ. Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2023 September 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:409424

IEEE

Đ. Božić Luburić, "Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:409424

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:409424

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Title	Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka
Title (english)	Determination of nonsteroidal anti-inflammatory drug residues and in vitro assessment of their ecotoxicological impact
Author	Đurđica Božić Luburić
Mentor	Ivana Radojčić Redovniković (mentor)
Mentor	Nina Bilandžić (komentor)
Committee member	Kristina Radošević (predsjednik povjerenstva)
Committee member	Višnja Gaurina-Srček (član povjerenstva)
Committee member	Ivana Varenina (član povjerenstva)
Committee member	Ivana Kmetič (član povjerenstva)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Cell Technology, Application and Biotransformations) Zagreb
Defense date and country	2022-03-25, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Universal decimal classification (UDC)	54 - Chemistry. Crystallography. Mineralogy
Abstract	U okviru doktorske disertacije razvijena je metoda za kvantitativno određivanje ostataka 27 nesteroidnih protuupalnih lijekova, kemijski različitih spojeva, tekućinskom kromatografijom ultra visoke djelotvornosti povezane s masenom spektrometrijom u uzorcima hrane životinjskog podrijetla (mlijeku, mišićnom tkivu) i uzorcima vode iz okoliša (otpadna voda). Optimiziran postupak ekstrakcije iz složenih matrica temelji se na enzimskoj hidrolizi koju slijedi ekstrakcija acetonitrilom, a dobiveni ekstrakt je pročišćen, uz istovremeno koncentriranje, ekstrakcijom na čvrstoj fazi. Validacijom metode ispitane su zadane značajke učinkovitosti koje su zadovoljile zakonski postavljene kriterije prihvatljivosti. Istraživanje je obuhvatilo i analizu realnih uzoraka s područja Republike Hrvatske, 449 uzoraka hrane životinjskog podrijetla i 96 uzoraka otpadnih voda. Kako bi se dobile informacije o toksičnosti tih spojeva koji dospijevaju u okoliš, in vitro testom na staničnim linijama ribe i ljudi ispitan je njihov citotoksični učinak.
Abstract (english)	In this dissertation, a method for the quantitative determination of 27 nonsteroidal anti-inflammatory drug residues by ultra high performance liquid chromatography – mass spectrometry in food of animal origin (milk, muscle) and environmental water (wastewater) was developed. Optimised sample processing consisted of enzymatic hydrolysis and acetonitrile extraction was followed by a clean-up of the extract by solid phase extraction. By validation of the method, verified performance characteristics complied with the legally set acceptability criteria. The study also included the analysis of the real samples from regions of the Republic of Croatia, 449 samples of food of animal origin and 96 samples of wastewater. In order to obtain information on the toxicity of these compounds that are released into the environment, their cytotoxic effect was examined using in vitro test on fish and human cell lines.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:409424
Promotion	2022
Study programme	Title: Postgraduate PhD Study in Nutrition Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
Type of resource	Text
Extent	133 str. ; 30 cm
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2022-11-09 12:46:17