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doctoral thesis
Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:351128
Kiš, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Quality Control
Laboratory for Food Quality Control

Cite this document

Kiš, M. (2020). Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:351128

Kiš, Maja. "Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:351128

Kiš, Maja. "Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:351128

Kiš, M. (2020). 'Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:351128

Kiš M. Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:159:351128

M. Kiš, "Učinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:351128

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Metadata
TitleUčinak gama zračenja i hladne plazme na smanjenje koncentracije T-2 i HT-2 toksina u žitaricama
Title (english)The effect of gamma irradiation and cold plasma on reduction of T-2 and HT-2 toxins in cereals
AuthorMaja Kiš
MentorJelka Pleadin (mentor)
Committee memberKsenija Markov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Quality Control)
(Laboratory for Food Quality Control)
Zagreb
Defense date and country2020-05-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Cilj ovog rada bio je ispitati pojavnost i omjere T-2 i HT-2 toksina u različitim žitaricama, uz praćenje klimatskih čimbenika, te učinak suvremenih fizikalnih metoda gama zračenja i hladne plazme na smanjenje njihove koncentracije, uz utvrđivanje utjecaja primijenjenih metoda na nutritivni sastav žitarica. U svrhu detekcije i kvantifikacije T-2 i HT-2 toksina razvijena je i validirana visoko osjetljiva potvrdna metoda tekućinske kromatografije u kombinaciji s masenom spektrometrijom (LC-MS/MS). Rezultati pokazuju značajnu pojavnost T-2 i HT-2 toksina u analiziranim žitaricama, primarno u zobi (70,0 %) te potom u ječmu (40,9 %), kukuruzu (26,8 %) i pšenici (19,2 %). Pritom je najveća utvrđena vrijednost sume T-2/HT-2 dobivena u kukuruzu iznosila 332,3 μg/kg. Smanjenje sadržaja T-2 i HT-2 toksina gama zračenjem ovisno je o brzini i primjenjenoj dozi zračenja, a postignuto je smanjenje koncentracije T-2 toksina od 51,3 % i HT-2 toksina od 56,6 %. Utjecaj hladne plazme na smanjenje koncentracije T-2 i HT-2 ovisi o vremenu izlaganja i radnom plinu. Nakon tretmana dušikovom plazmom s izvorom sinusoidalnog napona postignuta redukcija T-2 toksina iznosi 47,1 %, a HT-2 toksina 31,2 %. Za razliku od tretmana hladnom plazmom, primjena gama zračenja ne uzrokuje promjene u nutritivnom sastavu zobenog brašna.
Abstract (english)
Aim of this study was to investigate the occurrence and ratios of T-2 and HT-2 toxins in different cereals, with monitoring of climatic factors, and to establish the effect of modern physical methods, gamma irradiation and cold plasma, on reduction of T-2 and HT-2 toxins accompanied by monitoring of the influence of applied methods on nutritive composition of cereals. For this purpose, highly sensitive confirmatory liquid chromatography tandem-mass spectrometry (LCMS/ MS) method was developed and validated for the determination of T-2 and HT-2 toxins. The obtained results show significant occurence of T-2 and HT-2 toxins in analysed cereals, primarily in oats (70.0 %) and subsequently in barley (40.9 %), maize (26.8 %) and wheat (19.2 %). The maximal summary concentration of T-2/HT-2 was determined in maize (332.3 μg/kg). Reduction of T-2 and HT-2 toxins by gamma iraddiation is dependent on the radiation dose and dose rate applied, with maximal T-2 toxin reduction of 51.3% and HT-2 toxin of 56.6 % achieved. The effect of cold plasma on T-2 and HT-2 toxin reduction depends on exposure time and working gas type. After treatment with sinusoidal cold nitrogen plasma, the achieved maximal reduction of T-2 toxin was 47.1 % and HT-2 toxin 31.2 %. Unlike cold plasma treatment, gamma irradiation does not cause changes in the nutritive composition of oatmeal.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:351128
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
Extent167 str. : 30 cm
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2021-02-18 12:17:53

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