ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA

Mizdrak, Maja

prikaz prve stranice dokumenta ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA

doctoral thesis

ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA

KOHORTNA STUDIJA

Split: University of Split, School of Medicine, 2020. urn:nbn:hr:171:104947

Mizdrak, Maja

University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

Cite this document

APA 6th Edition

Mizdrak, M. (2020). ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA (Doctoral thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:104947

MLA 8th Edition

Mizdrak, Maja. "ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA." Doctoral thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:104947

Chicago 17th Edition

Harvard

Mizdrak, M. (2020). 'ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA', Doctoral thesis, University of Split, School of Medicine, accessed 28 January 2023, https://urn.nsk.hr/urn:nbn:hr:171:104947

Vancouver

Mizdrak M. ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA [Doctoral thesis]. Split: University of Split, School of Medicine; 2020 [cited 2023 January 28] Available at: https://urn.nsk.hr/urn:nbn:hr:171:104947

IEEE

M. Mizdrak, "ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA", Doctoral thesis, University of Split, School of Medicine, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:171:104947

Cite this item: https://urn.nsk.hr/urn:nbn:hr:171:104947

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Title	ZNAČAJ IZRAŽAJA DENDRINA, ČIMBENIKA RASTA VEZIVNOG TKIVA (CTGF) I c-Myb KAO RANIH POKAZATELJA KRONIČNE BUBREŽNE BOLESTI U PRIMARNIM GLOMERULOPATIJAMA : KOHORTNA STUDIJA
Title (english)	SIGNIFICANCE OF EXPRESSION OF DENDRIN, CONNECTIVE TISSUE GROWTH FACTOR AND c-Myb AS EARLY MARKERS OF CHRONIC KIDNEY DISEASE IN PRIMARY GLOMERULAR DISEASES : A COHORT STUDY
Author	Maja Mizdrak
Mentor	Merica Glavina Durdov (mentor)
Committee member	Mirna Saraga-Babić (predsjednik povjerenstva)
Committee member	Snježana Tomić (član povjerenstva)
Committee member	Gordana Đorđević (član povjerenstva)
Granter	University of Split School of Medicine (Department of Anatomy, Histology and Embryology) Split
Defense date and country	2020, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Pathology
Universal decimal classification (UDC)	616 - Pathology. Clinical medicine
Abstract	Cilj: Glomerularne bolesti su uzrok terminalnog stadija kronične bubrežne bolesti u 90% slučajeva. Podociti imaju centralnu ulogu u patogenezi glomerularnih bolesti i opadanju bubrežne funkcije. Ovisno o dužini i intenzitetu oštećenja, podociti hipertrofiraju i prolaze epitelijalno-mezenhimalnu pretvorbu ili umiru apoptozom. Glomeruloskleroza, intersticijska fibroza i tubularna atrofija su standardna obilježja KBB i predskazatelji bubrežnog ishoda, ali se traže rani prognostički biljezi. Dendrin je zdravom odraslom bubregu izražen na bazalnoj membrani podocita, a CTGF i c-Myb rijetko, ako ikad u podocitima i tubularnom epitelu. Cilj studije je analizirati njihov izražaj u GB i ispitati korelaciju s bubrežnom funkcijom u vrijeme biopsije i na kraju praćenja. Metode: Uzorak sadrži podatke o 48 ispitanika s primarnim GB dijagnosticiranim u razdoblju 1996.-2018. godine u Kliničkom bolničkom centru Split. Parafinski blokovi bubrežnog tkiva prikupljeni su iz arhive Odjela za patologiju, a klinički podaci iz povijesti bolesti Klinike za nefrologiju i Klinike za dječje bolesti. Urađena je imunohistokemijska analiza korištenjem protutijela na dendrin, c-Myb i CTGF. Izražaj dendrina je uspoređen između upalnih GB (IgGA/HSP) i podocitopatija (BMP i pFSGS), a izražaj CTGF i i c-Myb između IgA/HSP i kontrole. Izražaj svih biljega je uspoređen s laboratorijskim pokazateljima bubrežne funkcije u vrijeme biopsije i na kontroli. Rezultati: Medijan životne dobi je iznosio 32,5 godina (min-maks: 3-76) godina. Šesnaest (33%) ispitanika je bilo ženskog spola, a trideset i dva (67%) muškog spola. Broj dendrin pozitivnih jezgara je bio viši u podocitopatijama nego u IgAN i HSP. Uzorak izražaja dendrina definiran je kao membranski, dualni, nuklearni i negativni. Uzorak negativnog izražaja bio je povezan s nižim kontrolnim klirensom kreatinina. Medijan izražaja CTGF u citoplazmi tubularnog epitela je bio veći u IgAN/HSP nego u kontroli (P < 0,001). Nuklearni izražaj c-Myb u glomerulima i tubulima je bio veći u IgAN/HSP nego kontroli (P=0,005 i P=0,002). U praćenju, pad bubrežne funkcije bio je povezan s većim glomerularnim i tubularnim izražajem c-Myb, kao i CTGF (svi P < 0,05). Zaključak: Negativan izražaj dendrina i povećani izražaj c-Myb i CTGF su povezani s lošijom bubrežnom funkcijom na kraju praćenja te ih možemo smatrati novim, ranim prognostičkim pokazateljima progresivne KBB.
Abstract (english)	Aim: Glomerular diseases (GD) cause 90% of end-stage chronic kidney disease (CKD) cases. Podocytes have central role in pathogenesis of glomerular diseases and impaired renal function. Depending on the length and intensity of damage, they become hypertrophic, undergo epithelial-mesenchymal transition, or die. Glomerular sclerosis, as well as interstitial fibrosis and tubular atrophy, are good, but imperfect predictors of CKD and new early predictors are needed. In adult kidney, dendrin is normaly expressed in podocyte slit membrane and CTGF and c-Myb are rarely, if ever, expressed in podocytes and tubular cells. Aim of the study is to analyze their expression in GD and correlate with renal function after follow-up period. Methods: The sample contains data of 48 patients with primary GD (22 IgAN, 8 HSP, 7 BMP and 11 pFSGS cases) diagnosed in period 1996-2018 in University Hospital Centre of Split. Clinical data were collected from hospital records. Median of age was 32.5 (min-max: 3-76) years. Paraffin blocks of percutaneous renal biopsies were collected from Department of pathology. Immunohistochemistry/immunofluorescence was performed using antibodies to dendrin and c-Myb/CTGF, consecutively. Dendrin expression was compared between IgA/HSP and podocytopathies MCD and FSGS, and c-Myb and CTGF expression between IgA/HSP and controls - 12 patients who underwent nephrectomy due to renal cancer and had normal renal function. Further, the expresssion was correlated with laboratory parameters of renal function in time of the biopsy and at the end of follow-up. Results: Dendrin nuclear translocation occurs in different types of GD, including MCD, and dendrin expression presents in different patterns. Proportion of dendrin negative glomeruli correlated with lower creatinine clearance at the time of biopsy and at last follow-up (P=0.010 and P < 0.001, respectively). The cytoplasmic tubular CTGF expression was higher in IgAN/HSP than in controls (P < 0.001). The nuclear c-Myb expresssion in glomeruli and tubules was higher in IgAN/HSP than controls (P < 0.05). In the follow-up, decline in renal function correlated with glomerular and tubular c-Myb, as well as tubular CTGF expression (P < 0.05). Conclusions: The proportion of dendrin negative glomeruli, as well as upregulated c-Myb and CTGF expression, might be early indicators of late worse renal outcome.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:171:104947
Study programme	Title: Biology of Neoplasms Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	MEFST Repository
Created on	2021-09-06 11:02:26