Title Molekularna obilježja interneurona supragranularnih slojeva u frontalnoj kori mozga čovjeka Title (english) Molecular characteristics of supragranular layer interneurons in the human
frontal cortex Author Ivan Banovac Mentor Dora Sedmak (mentor)Mentor Zdravko Petanjek (komentor)Committee member Miloš Judaš (predsjednik povjerenstva)Committee member Nataša Jovanov Milošević (član povjerenstva)Committee member Goran Šimić (član povjerenstva)Granter University of Zagreb Defense date and country 2021-12-15, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Universal decimal classificationUDC ) 611 - Anatomy Abstract GABA-ergički kortikalni interneuroni raznovrsna su skupina neurona lokalnih neuronskih
krugova, a poznavanje njihovih molekularnih obilježja preduvjet je za razumijevanje složenih
kognitivnih funkcija i poremećaja koji se njima povezuju. Cilj ovoga istraživanja jest
usporediti svojstva kalretininskih, parvalbuminskih, kalbindinskih i somatostatinskih
interneurona između ventralnih (Brodmannovo polje 14r) i dorzalnih (Brodmannovo polje 9)
dijelova prefrontalne moždane kore čovjeka. Koristeći imunofluroescenciju s dvostrukim
obilježavanjem utvrđeni su udjeli pojedinih populacija interneurona u ukupnom broju neurona
te omjeri broja pojedinih populacija interneurona. RNAscope in situ hibridizacijom utvrđen je
neurotransmiterski fenotip pojedinih populacija interneurona. Ovim istraživanjem pokazano je
da se međusobni omjeri pojedinih populacija interneurona značajno razlikuju između
supragranularnih i infragranularnih slojeva, kao i unutar pojedinih supragranularnih slojeva
prefrontalne kore. Najbrojnija populacija su kalretininski neuroni čiji je udio u ukupnom broju
neurona značajno veći u Brodmannovom polju 14r (prosječno 10,24 %) nego u
Brodmannovom polju 9 (prosječno 8,21 %). Također, omjer kalretininskih i parvalbuminskih
neurona značajno je veći u Brodmannovom polju 14r (prosječno 1,62) nego u Brodmannovom
polju 9 (prosječno 1,15). Konačno, potvrđeno je da sve analizirane populacije interneurona
izražavaju GABA-ergičke molekularne biljege: vezikularni GABA transporter te glutamat
dekarboksilazu 65 i 67. Na temelju rezultata ovoga istraživanja može se zaključiti da postoje
značajne ventro-dorzalne razlike u udjelima pojedinih populacija interneurona u prefrontalnoj
kori čovjeka koje su najvećim dijelom posljedica povećanja udjela kalretininskih neurona u
ventralnim dijelovima prefrontalne kore.
Abstract (english) GABAergic cortical interneurons are a diverse group of local circuit neurons. Understanding
their molecular characteristics is a prerequisite for the comprehension of higher cognitive
functions and related disorders. The aim of this research is to compare the characteristics of
calretinin, parvalbumin, calbindin, and somatostatin interneurons between ventral (Brodmann
area 14r) and dorsal (Brodmann area 9) regions of the human prefrontal cortex. Double
labelling immunofluorescence was used to determine the proportion of each interneuron
population within the total neuron population as well as the relative ratios of individual
interneuron populations. The neurotransmitter phenotype of each interneuron population was
determined using RNA scope in situ hybridization. This research revealed that the ratios of
different interneuron populations differed significantly between supragranular and
infragranular cortical layers as well as within individual supragranular layers. Calretinin
neurons were the most numerous, with a significantly higher total proportion in Brodmann
area 14r (average: 10.24%) than in Brodmann area 9 (average: 8.21%). Furthermore, the ratio
between calretinin and parvalbumin neurons was significantly higher in Brodmann area 14r
(average: 1.62) than in Brodmann area 9 (average: 1.15). Finally, all analyzed interneuron
populations were confirmed to express GABAergic molecular markers: vesicular GABA
transporter and glutamate decarboxylase 65 and 67. In conclusion, there are significant
ventro-dorsal differences in the proportions of certain interneuron populations in the human
prefrontal cortex, which are predominantly the result of a significant increase of calretinin
neurons in the ventral prefrontal cortex.
Keywords
Parvalbumini
Kalbindin 2
Dorsolateralni prefrontalni korteks
Calbindins
Interneuroni
Hibridizacija in situ
Fluorescentna tehnika antitijela
Neurotransmiterska sredstva
Somatostatin
Fenotip
RNA
Keywords (english)
Parvalbumins
Calbindin 2
Dorsolateral Prefrontal Cortex
Calbindins
Interneurons
In Situ Hybridization
Fluorescent Antibody Technique
Neurotransmitter Agents
Somatostatin
Phenotype
RNA
Language croatian URN:NBN urn:nbn:hr:105:609164 Project Number: HRZZ IP- 2019-04-3182 Project Number: KK.01.1.1.01.0007 Study programme Title: Neuroscience Type of resource Text Extent 168 str. File origin Born digital Access conditions Open access Terms of use Repository Dr Med - University of Zagreb School of Medicine Digital Repository Created on 2022-01-27 09:49:14
