Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1

Šemanjski, Kristina

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doctoral thesis

Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1

Zagreb: University of Zagreb, School of Medicine, 2021. urn:nbn:hr:105:414026

Šemanjski, Kristina

University of Zagreb
School of Medicine

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APA 6th Edition

Šemanjski, K. (2021). Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1 (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:414026

MLA 8th Edition

Šemanjski, Kristina. "Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1." Doctoral thesis, University of Zagreb, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:105:414026

Chicago 17th Edition

Harvard

Šemanjski, K. (2021). 'Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1', Doctoral thesis, University of Zagreb, School of Medicine, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:105:414026

Vancouver

Šemanjski K. Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1 [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2021 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:105:414026

IEEE

K. Šemanjski, "Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:105:414026

Cite this item: https://urn.nsk.hr/urn:nbn:hr:105:414026

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Title	Spolni dimorfizam izvanorbitalnih suznih žlijezda u miševa bez gena SF-1
Title (english)	Sexual Dimorphism of the Extraorbital Lacrimal Glands in Sf-1 Knockout Mice
Author	Kristina Šemanjski
Mentor	Davor Ježek (mentor)
Committee member	Floriana Jakuš (predsjednik povjerenstva)
Committee member	Gordana Jurić-Lekić (član povjerenstva)
Committee member	Romana Gračan (član povjerenstva)
Granter	University of Zagreb School of Medicine Zagreb
Defense date and country	2021, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Cytology, Histology and Embryology
Universal decimal classification (UDC)	61 - Medical sciences
Abstract	Spolni dimorfizam (SD) kao razlika u karakteristikama mužjaka i ženki iste vrste poznat je i u suznoj žlijezdi, a postoje i podaci o utjecaju testosterona na SD. U nokaut-miševima za steroidogeni faktor 1 (Sf-1 KO) ne razvijaju se gonade. Cilj doktorskog rada bio je istražiti pojavu spolnog dimorfizma žlijezde Sf-1 KO mužjaka i ženki miševa te suzne žlijezde Sf-1 KO mužjaka koji su dobivali nadomjesnu testosteronsku terapiju (TP). Provedena je detaljna histološka analiza i imunohistokemijska analiza izražaja androgenih receptora (AR) uz stereološku kvantifikaciju i statističku analizu rezultata. Nije bilo statistički značajne razlike u srednjem volumenu acinusa, vezivnog tkiva ni duljine odvodnih kanala između mužjaka, ženki kao ni mužjaka na nadomjesnoj terapiji testosteronom. Stanice acinusa mužjaka koji su dobivali TP bile su pozitivne na androgene receptore, a u mužjaka bez primjene TP signal je bio negativan. Davanje TP je induciralo pojavu AR, ali nije dokazan utjecaj na morfologiju. Može se zaključiti da spolni dimorfizam suzne žlijezde Sf-1 KO miševa različitog genetskog spola nije prisutan, pa spolni hormoni imaju presudnu ulogu u razvoju spolnog dimorfizma suzne žlijezde.
Abstract (english)	Sexual dimorphism (SD), a difference in the characteristics of males and females of the same species was found in lacrimal glands together with data on the influence of testosterone. Knockout mice for steroidogenic factor 1 (Sf-1 KO) do not develop gonads. We aimed to investigate the lacrimal gland sexual dimorphism in male and female Sf-1 KO mice and the lacrimal glands of Sf-1 KO males receiving replacement testosterone therapy (TP). Detailed histological analysis and immunohistochemical analysis of androgen receptor expression (AR) were carried out with stereological quantification and statistical analysis of the results. There was no statistically significant difference in the mean volume of acini, connective tissue or ductal system length between males and females nor males on testosterone replacement therapy. TP induced androgen receptor in male acini-cells, and in males without TP administration, the signal was negative. Administering TP induces the occurrence of AR, but no impact on morphology has been demonstrated. It can be concluded that the sexual dimorphism of the lacrimal gland in Sf-1 KO mice of different genetic sex is not present, so sex hormones play a crucial role in the development of sexual dimorphism in the lacrimal glands.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:105:414026
Promotion	2021
Project	Number: KK.01.1.1.01.0008 Title: Reproduktivna i regenerativna medicina - istraživanje novih platformi i potencijala Leader: Ana Katušić Bojanac Jurisdiction: Croatia Funder: MZO Funding stream: ZP
Study programme	Title: Biomedicine and Health Sciences Study programme type: university Study level: postgraduate Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resource	Text
Extent	161 str.
File origin	Born digital
Access conditions	Open access
Terms of use
Public note	Sumentor: Gregor Majdič
Repository	Dr Med - University of Zagreb School of Medicine Digital Repository
Created on	2021-08-02 09:19:14

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