Aim: to examine the effect of educational intervention on the quality of life of patients in all stages of chronic venous disease (CVD) under the care of general practitioners. Materials and methods: 122 patients with CVD were randomly divided into intervention group [N=62 (46 women)] and control group [N=60 (45 women)]. The intervention group received education on CVD, while the control group did not. Both groups received equal treatment in accordance with the CVD treatment protocol. Monitoring period lasted for 6 months, the quality of life was assessed with the CIVIQ questionnaire at the beginning of the treatment, as well as after 2 and 6 months. Results: The groups had no differences in relation to the parameters assessed at baseline. All monitored patients had an increase in the quality of life during treatment, as well as on physical, psychological and pain dimensions. There were no differences in the quality of life between patient groups in terms of received education on CVD. Patients in the intervention group that received sclerotherapy showed higher quality of life in comparison to other patients in the same group. After 2 months of treatment, all patients with wounds showed improvement in healing and their quality of life reached high level. The educational intervention received an excellent grade with the participants showing interest to continue. The Croatian version of CIVIQ-20 questionnaire proved to be unreliable, while CIVIQ-14 proved to be a reliable and valid instrument. Conclusion: A one-time educational intervention on CVD had no greater influence on the improvement of the quality of life of patients in the intervention group than the standard treatment of both groups had on improving the quality of life overall and by dimensions. To assess the quality of life of patients with CVD, the shortened version of CIVIQ-14 questionnaire is recommended.