Artistic gymnastics is divided into menʼs and womenʼs gymnastics which basically differ according to the number and type of competition disciplines. Menʼs gymnastics consist of six apparatus (floor, pommel horse, rings, vault, parallel bars, and horizontal bar) and four womenʼs apparatus (vault, uneven bars, beam, and floor) of all-around competition in artistic gymnastics. This research aimed to determine the differences in kinematic parameters of the Tsukahara entry on the vault between gymnasts (menʼs and womenʼs). The sample consisted of elite men (N = 5) and women (N = 5) gymnasts, who performed jumps at the qualifications and finals of the FIG World Challenge Cup 2017 in Osijek, Croatia. The sample of variables consisted of 35 kinematic parameters of Tsukahara entry on vault in women (Tsukahara stretched with 1/1 turn (360°) off) and men (Driggs). Data processing and kinematic 3D analysis were performed by the APAS (Ariel Performance Analysis System, 2016). Normality of distribution was determined by the Shapiro-Wilk test. Gender differences in kinematic variables were calculated by univariate analysis of variance (ANOVA) with Bonferroni correction on p<0.05. Obtained results showed that there were no statistically significant differences between the genders in the variables: the length of the last step, the angle of the center of gravity to the floor at the last touch of the foot with the springboard, the distance between the palms in the first contact with the apparatus. The duration of the take-off from the apparatus was equal in both genders and there are no differences in hip angles (left and right) at the first contact of hands with the apparatus. Statistically, a significant difference was found in the left hip angle at the first contact with a springboard, where the men had a smaller angle than the women. Results of the variable: center of mass to the axis of the apparatus in the first contact of the hands with the apparatus showed a significant difference where the women have a greater angle than the men. The angle of the left elbow in the first contact of the palms with the apparatus proved to be statistically significant and is higher in men, which means that the women had the first contact with the apparatus with a bent left elbow. From the results of the second flight, there is a statistically significant difference between men and women at the center of gravity regarding the axis of the apparatus up to the maximum flight height. The angle of the center of gravity with the floor at the first contact of the foot with the floor is statistically significant and distinguishes the genders and is higher in men. There is also a statistically significant difference in the angle variables: left and right knee joints angle at the first contact of the foot with a mat which is greater in the women and the left and the right hip joint angle at the first contact of the foot with the mat which is higher in the men. Based on the obtained results it can be concluded that gender differences are visible in the second flight phase and take-off from the apparatus. The knowledge gained show us the differences or similarities between the performance techniques of the jumps and it is considered that gymnasts have spaces for improvement and further jumps upgrading.